Journal: Molecular Cell

Article Title: POINT technology illuminates the processing of polymerase-associated intact nascent transcripts

doi: 10.1016/j.molcel.2021.02.034

Figure Lengend Snippet:

Article Snippet: The isolated RNA was fragmented to 150-200 nt at 90°C for 10 min according to a protocol of NEBNext Ultra II Directional RNA library prep kit to prepare PCR library.

Techniques: Sequencing, cDNA Library Assay, Software

Journal: Journal of Clinical Microbiology

Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis : a Tool for Prediction of Virulence

doi: 10.1128/JCM.02464-16

Figure Lengend Snippet: Results of the pathotyping multiplex on a subset of H. parasuis isolates from the additional isolate collection. M, Quick-load 100 bp DNA ladder.

Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

Techniques: Multiplex Assay

Journal: Journal of Clinical Microbiology

Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis : a Tool for Prediction of Virulence

doi: 10.1128/JCM.02464-16

Figure Lengend Snippet: Negative-control panel showing specificity of the primer sets across a range of commensal and pathogenic bacteria of the pig respiratory tract (Haemophilus parasuis positive controls serovar 2 (SW140) and serovar 5 (Nagasaki), Actinobacillus minor, A. porcinus, A. indolicus, Streptococcus suis, A. pleuropneumoniae, and Bordetella bronchiseptica). M, Quick-load 100 bp DNA ladder.

Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

Techniques: Negative Control

Journal: Journal of Clinical Microbiology

Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis : a Tool for Prediction of Virulence

doi: 10.1128/JCM.02464-16

Figure Lengend Snippet: Limit of detection for the pathotyping multiplex PCR using pure genomic DNA (genomes/μl) for seven isolates representing all of the bands present in the pathotyping multiplex PCR. M, Quick-load 100 bp DNA ladder.

Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

Techniques: Multiplex Assay